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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two extra splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best effects are...
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This manual outlines more possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which more info lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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